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晚期糖基化终产物是一组由不同种类物质组成的复杂混合物,其与很多疾病相关,如糖尿病及其并发症、肾衰竭以及与老龄化相关等疾病。人血清白蛋白(HSA)是血清中含量最丰富的蛋白质。葡萄糖对血液中的HSA和其它蛋白质的结构具有修饰作用,发生糖基化反应。本文利用LC/MS联用技术,通过蛋白质组学方法,寻找差异肽段,研究糖基化HSA的修饰位点以及修饰种类,对于进一步寻找临床诊断糖尿病以及与老龄化相关疾病的肽类生物标志物具有重要意义。
Advanced glycation end products are a complex mixture of different substances that are associated with many diseases such as diabetes and its complications, kidney failure, and aging-related diseases. Human serum albumin (HSA) is the most abundant serum protein. Glucose has a modifying effect on the structure of HSA and other proteins in the blood and a glycosylation reaction takes place. In this paper, the use of LC / MS technology, through proteomics method to find the difference between the peptide to study glycosylation HSA modified sites and modified species, for further search for clinical diagnosis of diabetes and aging-related diseases peptide biomarkers Material is of great importance.