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Antibacterial action of 4-[p-acetylamino benzenesul fonamido] selenomorpholine (SeB) was studied by microcalorimetry with an LKB-2277 Bioactivity Monitor. It was found that SeB had the capacity to inhibit the metabolic growth of Escherichia coll But in different solvent, the extent of inhibition is different. Removing the effect of solvent, we obtained the half-inhibition concentration(IC50) of SeB fromthe power-time curves. The value of IC50 of SeB was 1319μg · mL- 1.