建立了测定抗真菌制剂M18中吩嗪 1 羧酸的反相高效液相色谱方法。流动相为甲醇 5mmol/L磷酸缓冲液(pH 5 0 ) (体积比为 6 0∶40 ) ,流速为 1mL/min ,检测波长为 2 48nm ,线性范围是 5 0mg/L～ 5 0 0mg/L ,检测限是 30mg/L ,回收率为 97 5 3% ,RSD为 1 5 %。该方法具有快速、简便、灵敏、重现性好的特点 A reversed-phase high performance liquid chromatographic method was established for the determination of the phenazine 1 carboxylic acid in the antimycotic M18. The mobile phase was methanol 5mmol / L phosphate buffer (pH 50) (volume ratio 60:40), the flow rate was 1mL / min, the detection wavelength was 2 48nm, the linear range was 50mg / L ~ 500mg / L , The detection limit is 30mg / L, the recovery rate is 97 5 3%, RSD is 15%. The method is fast, simple, sensitive and reproducible