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背景:碱性成纤维细胞生长因子可促进体外培养的神经元存活及突起生长,拮抗兴奋性氨基酸毒性,对中枢神经系统功能恢复起重要作用,能否通过影响脑细胞内游离钙离子浓度对缺血脑组织起保护作用。目的:从细胞水平探讨碱性成纤维细胞生长因子对大鼠局灶性脑缺血再灌注损伤时神经细胞内游离Ca2+浓度变化的影响。设计:完全随机对照实验。单位:郑州大学第二附属医院神经内科。材料:实验于2003-08/12在郑州大学第二附属医院神经内科实验室完成。24只SD大鼠随机分为假手术组、缺血再灌注组和碱性成纤维细胞生长因子治疗组,每组8只。方法:缺血再灌注组及碱性成纤维细胞生长因子治疗组采用线栓法建立大脑中动脉闭塞模型;假手术组除不插线外,余同其他两组。碱性成纤维细胞生长因子治疗组于缺血后即刻腹腔注射10μg/kg碱性成纤维细胞生长因子,其余两组腹腔注射等量生理盐水。各组大鼠于缺血再灌注24h检测脑细胞游离钙浓度。主要观察指标:各组大鼠缺血再灌注24h脑细胞游离钙浓度。结果:24只大鼠全部进入结果分析。缺血再灌注组明显高于假手术组犤(673.46±18.44),(224.71±10.58)nmol/L,(F=1329.06,P<0.01)犦,碱性成纤维细胞生长因子治疗组明显低于缺血再灌注组犤(378.37±21.08),(673.46±18.44)nmol/L(F=1329.06,P<0.01)犦。结论:碱性成纤维细胞生长因子能明显抑制大鼠缺血再灌注后脑组织内游离钙水平,起到稳定细胞膜,防止细胞内钙超载的作用。
BACKGROUND: Basic fibroblast growth factor (BfF) can promote the survival and growth of neurons in vitro and antagonize the excitotoxicity of amino acids. It plays an important role in the recovery of central nervous system function. Whether it can influence the concentration of free calcium ion in brain cells Blood brain tissue plays a protective role. OBJECTIVE: To investigate the effect of basic fibroblast growth factor (FGF) on the changes of intracellular free Ca2 + concentration during focal cerebral ischemia / reperfusion injury in rats at the cellular level. Design: Complete randomized controlled experiment. Unit: Second Affiliated Hospital of Zhengzhou University, Department of Neurology. MATERIALS: The experiment was performed at the Department of Neurology, Second Affiliated Hospital of Zhengzhou University from August to December 2003. Twenty-four SD rats were randomly divided into sham operation group, ischemia reperfusion group and basic fibroblast growth factor treatment group, with 8 rats in each group. Methods: The model of middle cerebral artery occlusion (MCAO) was established by ischemia-reperfusion group and basic fibroblast growth factor (FGF) -treated group. The sham operation group was the same as the other two groups. In the basic fibroblast growth factor group, intraperitoneal injection of 10μg / kg basic fibroblast growth factor was performed immediately after ischemia, and the other two groups were injected intraperitoneally with the same amount of normal saline. Rats in each group were subjected to ischemia-reperfusion for 24 hours to detect free calcium concentration in brain cells. MAIN OUTCOME MEASURES: Free calcium concentration in brain cells of rats in 24h after ischemia-reperfusion. Results: All 24 rats entered the result analysis. Compared with the sham-operation group, the level of NF-κB in ischemia-reperfusion group was significantly higher than that in the sham operation group (673.46 ± 18.44, 224.71 ± 10.58 nmol / L, F = 1329.06, P <0.01) Ischemia-reperfusion group 犤 (378.37 ± 21.08), (673.46 ± 18.44) nmol / L (F = 1329.06, P <0.01) 犦. Conclusion: Basic fibroblast growth factor can significantly inhibit the level of intracellular calcium in rats after ischemia-reperfusion, stabilize the cell membrane and prevent the overload of intracellular calcium.