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目的:初步探讨诱骗受体3(Dc R3)在复发性流产(RSA)中可能的作用机制。方法:ELISA方法检测55例复发性流产(RSA)患者(研究组)及35例非意愿妊娠正常妊娠、自愿要求人工流产孕妇(对照组)血清中Dc R3、白介素(IL)-2、IL-10水平;免疫组织化学方法检测19例研究组及16例对照组患者流产蜕膜组织中Dc R3的表达。结果:研究组血清中Dc R3、IL-10水平显著低于对照组(P<0.05);IL-2水平显著高于对照组(P<0.05)。蜕膜中Dc R3免疫阳性产物定位于细胞质和细胞膜,Dc R3在研究组中的表达显著低于对照组(P<0.05)。结论:Dc R3在RSA患者中低表达,可能是打破母-胎免疫耐受平衡的原因之一。
Objective: To investigate the possible mechanism of decoy receptor 3 (Dc R3) in recurrent spontaneous abortion (RSA). Methods: The serum levels of DcR3, IL-2 and IL-6 in 55 recurrent spontaneous abortion (RSA) patients (study group) and 35 non-consensual pregnant normal pregnancies were determined by ELISA. 10 levels. Immunohistochemistry was used to detect the expression of DcR3 in decidua of 19 patients in study group and 16 patients in control group. Results: The serum levels of DcR3 and IL-10 in the study group were significantly lower than those in the control group (P <0.05). The level of IL-2 in the study group was significantly higher than that in the control group (P <0.05). Dc R3 immunopositive products in the decidua were localized in the cytoplasm and cell membrane, and the expression of Dc R3 in the study group was significantly lower than that in the control group (P <0.05). Conclusion: The low expression of Dc R3 in patients with RSA may be one of the reasons to break the balance of maternal-fetal immune tolerance.