论文部分内容阅读
目的 观察分析新建立的人肝癌转移细胞系 (ＭＨＣＣ97)转移相关因子ｍＲＮＡ和蛋白表达水平 ,以探讨其转移的可能机制。方法 利用裸鼠人肝癌转移模型 (ＬＣＩ Ｄ2 0 )的瘤组织 ,通过体外培养获得首株高转移人肝癌细胞系 ,除对其基本生物学特性进行观察外 ,还采用逆转录 聚合
Objective To observe the mRNA and protein expression of metastasis related factors in newly established human hepatoma metastasis cell line (MHCC97) to explore the possible mechanism of metastasis. Methods The first strain of highly metastatic human hepatocellular carcinoma cell line was obtained by in vitro culture of human liver cancer metastasis model of nude mice (LCI D2 0). In addition to the observation of its basic biological characteristics, reverse transcriptase polymerization