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采用添加NaH_2PO_4的方法调节猪场污水中氮磷比(N∶P)分别为8∶1、16∶1、32∶1和64∶1,以未添加NaH_2PO_4的污水为对照(氮磷比为532∶1),探讨一株耐污绿球藻(Chlorococcum sp.)在不同氮磷比污水中的生长性能及其对猪场污水(初始氨氮浓度为291.31 mg·L~(-1))的净化效果.结果表明:经过12 d的培养,绿球藻(接种密度为400×104cells·m L~(-1))在N∶P为64∶1的污水中生长最好,且对污水中氨态氮和总氮的去除效果最佳,细胞密度和生物量分别为3393×104cells·m L~(-1)和0.49 g·L~(-1),对氨态氮和总氮的去除率分别为74.94%和48.78%,显著高于对照组,氨态氮浓度降低到73.01 mg·L~(-1),总氮浓度降低到148.96 mg·L~(-1).培养期间各试验组污水中硝态氮浓度均升高.培养12 d后,N∶P为64∶1组污水中总磷浓度降低为3.07 mg·L~(-1),去除率为71.86%.综上,绿球藻在N∶P为64∶1的污水中生长性能及其对污水中氨态氮和总氮的去除效果均最佳,可使污水中的氨态氮和总磷浓度基本达到相关排放标准.
The method of adding NaH 2 PO 4 was used to adjust the ratio of nitrogen to phosphorus (N: P) in the wastewater of pig farms to 8:1, 16:1, 32:1 and 64:1, respectively, with no addition of NaH 2 PO 4 as control : 1) to investigate the growth performance of a strain of Chlorococcum sp. In different N / P ratios of sewage and to purify the wastewater (initial ammonia nitrogen concentration of 291.31 mg · L -1) The results showed that Chlorococcum sp. (Inoculum density 400 × 104cells · m L -1) grew best in the wastewater with N:P of 64:1 after 12 days culture, The removal efficiency of total nitrogen and total nitrogen was the best, and the cell density and biomass were 3393 × 104cells · m L -1 and 0.49 g · L -1, respectively. The removal rates of ammonia nitrogen and total nitrogen Were 74.94% and 48.78% respectively, which were significantly higher than that of the control group, the concentration of ammonia nitrogen decreased to 73.01 mg · L -1, and the total nitrogen concentration decreased to 148.96 mg · L -1. The concentration of nitrate nitrogen in wastewater was increased after 12 days incubation, the total phosphorus concentration in wastewater with N:P of 64:1 decreased to 3.07 mg · L -1, and the removal rate was 71.86% Growth performance of Chlorella vulgaris in sewage with N: P of 64:1 and its effect on ammonia nitrogen and total nitrogen in wastewater Other effects are the best, can Ammonium and total phosphorus in wastewater substantially meet the relevant emission standard.