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目的:研究淫羊藿苷对体外培养大鼠股骨组织(骨干和骨骺端)吸收活性的影响。方法:体外分离培养大鼠股骨组织的骨干和骨骺端,48 h后采用终浓度为1×10-5mol·L-1的淫羊藿苷对体外培养骨干和骨骺端进行处理。测定抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,StrACP)活性;测定培养基中葡萄糖(glucose,Glu)和乳酸(lactic acid,Lac);RealTime RT-PCR检测抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,StrACP)、集落刺激因子(macrophage colony stimulating factor,MCSF),组织激酶K(cathepsin K,CTSK)mRNA表达水平。结果:1×10-5mol·L-1淫羊藿苷可抑制StrACP活性,增加培养基中乳酸含量和减少培养基中葡萄糖含量,抑制StrACP,MCSF,CTSK mRNA的表达水平。结论:淫羊藿苷抑制体外培养大鼠股骨组织的骨骺端和骨干吸收活性。
Objective: To study the effect of icariin on the absorption activity of the femoral bone tissue (the trunk and metaphysis) in vitro. Methods: The backbone and metaphysis of rat femur tissue were isolated and cultured in vitro. After 48 h, icariin with the final concentration of 1 × 10-5mol·L-1 was used to treat the stem and metaphysis in vitro. The activity of tartrate-resistant acid phosphatase (StrACP) was assayed. Glucose (Glu) and lactic acid (Lac) were determined in the medium. The tartrate-resistant acid phosphatase (StrACP), macrophage colony stimulating factor (MCSF) and cathepsin K (CTSK) mRNA. Results: 1 × 10-5mol·L-1 icariin could inhibit the activity of StrACP, increase the content of lactic acid in the medium and decrease the glucose content in the medium, and inhibit the expression of StrACP, MCSF and CTSK mRNA. CONCLUSION: Icariin can inhibit the metaphyseal end and the bone resorption activity of rat femur in vitro.