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采用分光光度法测定注射用复方维生素(3)中微量草酸的方法。在氯化钾-盐酸缓冲液下,Fe3+和草酸的络合物能使Fe3+与磺基水杨酸的紫色络合物颜色变浅,测定显色反应后溶液的吸光度,试验中优化了显色反应时间、显色剂用量。结果显示,显色溶液最大吸收波长为503nm,草酸在0.006—0.036mg/mL浓度范围内,其吸光度与浓度呈良好线性关系,y=-17.552x+1.0283,r=0.9991。样品加样回收率为97.6%。本方法能准确检查注射用复方维生素(3)中微量草酸的含量。
Determination of trace oxalic acid in compound vitamin (3) for injection by spectrophotometry. Under potassium chloride-hydrochloric acid buffer, the complex of Fe3 + and oxalic acid makes the purple complex of Fe3 + and sulfosalicylic acid lighter, and the absorbance of the solution after the color reaction is measured, and the color development is optimized Reaction time, developer dosage. The results showed that the maximum absorption wavelength of chromogenic solution was 503nm, and the absorbance of oxalic acid showed a good linear relationship with the concentration of 0.006-0.036mg / mL, y = -17.552x + 1.0283, r = 0.9991. The sample recovery was 97.6%. The method can accurately check the content of trace oxalic acid in compound vitamin (3) for injection.