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葡萄牙海岸松被松材线虫感染后的迅速死亡表明有其他生物的介入。内生细菌是一类普遍存在的生物,它在大部分植物中能形成种群。松材线虫携带的细菌也有可能导致海岸松的迅速死亡。笔者通过培养分离得到的细菌分离物,利用扩增性核糖体DNA限制性酶切片段分析(ARDRA)和变性梯度凝胶电泳分析(DGGE),了解被感染的和未被感染的海岸松内生细菌的种群结构,以分析鉴定与松材线虫相关的细菌。从样树和松材线虫中分离的细菌Alphaproteobacteria、Bacteroidetes属仅存在于内生菌中,而Actinobacteria、Firmicutes被发现仅与松材线虫相关。ARDRA和DGGE分析证明内生菌种群结构多样性的差异可能与松材线虫的入侵有关。这种细菌种群的多样性差异似乎与Burkholderia、Pseudomonas和Luteibacter属中的某些菌株有关。而从树中分离出来的Janthinobacterium agaricidamnosum、P.lutea和Dyella yeojuensis与松材线虫相关。
The rapid death following pine wood nematode infection on the coast of Portugal suggests the involvement of other organisms. Endophytic bacteria are a type of ubiquitous organism that forms a population in most plants. Bacteria that are carried by pine wood nematodes may also cause the rapid death of coast pine. By culturing the isolated bacterial isolates, we used amplified ribosomal DNA restriction fragment analysis (ARDRA) and denaturing gradient gel electrophoresis (DGGE) to understand the relationship between infected and uninfected Coastal pine endophytes Bacterial population structure was analyzed to identify bacteria associated with pine wood nematode. The bacteria Alphaproteobacteria, Bacteroidetes, isolated from the sample tree and the pine wood nematode, are found only in endophytes, while Actinobacteria, Firmicutes, are found to be associated only with pine wood nematodes. ARDRA and DGGE analysis showed that the difference in endogamy population structure diversity may be related to the invasion of pine wood nematode. The diversity of this bacterial population appears to be related to certain strains of the genus Burkholderia, Pseudomonas and Luteibacter. Janthinobacterium agaricidamnosum, P.lutea and Dyella yeojuensis isolated from the tree are associated with pine wood nematodes.