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目的探讨幽门螺杆菌（Ｈｐ）感染对正常胃粘膜向胃癌演进过程中对胃上皮细胞增殖的影响。材料和方法采用免疫组化染色方法测定胃粘膜增殖细胞核抗原（ＰＣＮＡ）标记指数（ＬＩ）。１１例基本正常胃粘膜、３２例浅表性胃炎、８３例萎缩性胃炎伴肠化、２５例异型增生和１０例胃癌被纳入本研究。结果１．从正常胃粘膜、浅表性胃炎、萎缩性胃炎伴肠化、异型增生至胃癌，胃粘膜上皮细胞ＰＣＮＡ标记指数进行性地升高，各阶段病变组之间及病变组与正常对照组相比均有显著差异（Ｐ＜０．０１）；２．浅表性胃炎组和萎缩性胃炎伴肠化轻度组中Ｈｐ阳性病例的ＰＣＮＡ标记指数分别为１３．１４±１．６和１９．６８±２．２２，而Ｈｐ阴性病例则为６．９５±０．７８和１１．３４±１．８９，有显著差异（Ｐ＜０．０１）；其他各组中Ｈｐ阳性与阴性病例间ＰＣＮＡ标记指数无显著差异（Ｐ＞０．０５）。结论Ｈｐ感染增加浅表性胃炎和轻度萎缩性胃炎阶段胃粘膜上皮细胞增殖，Ｈｐ感染可能在胃癌发生过程中的早期阶段参与启动作用。
Objective To investigate the effect of Helicobacter pylori (Hp) infection on the proliferation of gastric epithelial cells during the evolution of normal gastric mucosa to gastric cancer. Materials and Methods The gastric mucosal proliferating cell nuclear antigen (PCNA) labeling index (LI) was measured by immunohistochemical staining. 11 cases of normal gastric mucosa, 32 cases of superficial gastritis, 83 cases of atrophic gastritis with intestinal metaplasia, 25 cases of dysplasia and 10 cases of gastric cancer were included in this study. Results 1. From the normal gastric mucosa, superficial gastritis, atrophic gastritis with intestinal metaplasia, dysplasia to gastric cancer, gastric mucosal epithelial cells PCNA labeling index increased in stages of disease groups and lesions compared with the normal control group All have significant difference (P <0.01); 2. The PCNA labeling index of Hp positive cases in superficial gastritis group and atrophic gastritis with mild intestinal metaplasia were 13.14 ± 1.6 and 19.68 ± 2.22, respectively, while those in Hp negative cases were 6.95 ± 0.78 and 11.34 ± 1.89, respectively (P <0.01). There was no significant difference in PCNA labeling index between Hp positive and negative cases in other groups (P> 0.05). Conclusion Hp infection can increase the proliferation of gastric mucosal epithelial cells in superficial gastritis and mild atrophic gastritis. Hp infection may be involved in the early stage of gastric carcinogenesis.