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为探讨快速而准确的检测香蕉束顶病毒的方法，采用本所制备的香蕉束顶病毒（Ｂａｎａｎａｂｕｎｃｈｙｔｏｐｖｉｒｕｓ－ＢＢＴＶ）多克隆抗体ＩｇＧ和台湾的香蕉束顶病毒单克隆抗体２Ｈ６，用酶联免疫吸附测定技术（ＥＬＩＳＡ）双抗体夹心法（ＤＡＳ）测定了漳州市天宝的香蕉束顶病病株。试验结果表明，多抗和单抗相结合的ＤＡＳ－ＥＬＩＳＡ法能检测到病组织汁液的最大稀释度为１∶６４０，而单独使用单抗的最大稀释度为１∶１０。同时采用多抗和单抗相结合的ＤＡＳ－ＥＬＩＳＡ法比较了病株根、假茎、叶片中脉、叶肉的测定结果，假茎和中脉的病毒含量高于叶肉，根未检出。用此法能检测到蚜虫体内的病毒。
In order to explore a rapid and accurate method for the detection of banana bunchy top virus, Bananabunchytopvirus-BBTV polyclonal antibody IgG and Taiwan banana bunchy top monoclonal antibody 2H6 were prepared by enzyme-linked immunosorbent assay Technology (ELISA) double antibody sandwich method (DAS) was used to determine banana bunchy top disease in Tianbao, Zhangzhou. The results showed that the combination of polyclonal antibody and monoclonal antibody DAS-ELISA method can detect the maximum dilution of diseased juice was 1: 640, and the maximum dilution of monoclonal antibody alone was 1:10. In the meantime, DAS-ELISA method was used to compare the results of the determination of midrib, midrib and leaf mesophyll of diseased plants with DAS-ELISA. The virus content in pseudostem and midrib was higher than that in mesophyll and the roots were not detected. Using this method can detect aphids in vivo virus.