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采用反相高效液相色谱系统,在LichrosorbRP-C18色谱柱上,甲醇-水(68:32)为流动相,215nm为检测波长,井以扑米酮为内标物,对吡喹酮团进行了含量测定。在所测20~100ug/ml浓度范围内线性良好,r=0.9997。
The reversed-phase high performance liquid chromatography (HPLC) was performed on Lichrosorb RP-C18 column using methanol-water (68:32) as the mobile phase and 215 nm as the detection wavelength. The content determination. Linearity was good at concentrations ranging from 20 to 100 ug / ml, r = 0.9997.