为了研究侵袭蛋白与免疫保护的关系 ,为志贺菌苗的研制提供理论依据。经志贺菌苗角结膜免疫豚鼠 ,毒株攻击 ,观察侵袭蛋白对免疫保护的影响 ,观察到表达侵袭蛋白的菌苗较不表达侵袭蛋白的菌苗对毒株攻击有较高的保护率 ;用BA ELISA方法检测免疫后豚鼠泪液中特异性抗体水平 ,其结果较对照显著升高。说明侵袭蛋白的表达有一定的增加菌苗的免疫保护作用 In order to study the relationship between invasion protein and immune protection, provide a theoretical basis for the development of Shigella. Shizuoka vaccine seedling conjunctiva immune guinea pigs, strains of attack, observe the impact of invasion of protein on immune protection, the expression of the invasion of the protein vaccine than those that do not express the invasion of the vaccine against the virus strains have a higher protection rate; The level of specific antibody in guinea pig lacrimal fluid after immunization was detected by BA ELISA. The result was significantly higher than that of the control. Indicating that the expression of the invasion of a certain increase in vaccine immune protection