论文部分内容阅读
应用形态学、细胞学和分子标记的方法对通过胚挽救获得的青花菜与萝卜属间杂种植株进行了鉴定。结果表明:杂种植株的生长势明显强于父本和母本,大部分形态性状超出了父、母本的范围,部分性状介于父母本之间或偏向于父本或母本一方。表型和细胞学观察显示杂种植株具有类似母本的雄性不育特性。SSR分子标记分析表明,杂种植株包含了双亲的遗传信息。可见,远缘杂种是双亲间的真杂种。远缘杂交种的获得与初步鉴定为促进萝卜属和芸薹属的基因交流提供了桥梁种质,为进一步创制萝卜或芸薹属作物新种质提供了基础材料。
Morphological, cytological and molecular markers were used to identify the hybrid plants between broccoli and radish obtained through embryo rescue. The results showed that the growth vigor of the hybrid plants was significantly stronger than that of the male parent and the female parent, most of the morphological traits exceeded the range of the parent and the female parent, and some of the traits were between the parents or the parents. Phenotypic and cytological observations show that hybrid plants have maternal-like male sterility traits. SSR molecular marker analysis showed that hybrid plants contain the genetic information of parents. Can be seen that distant hybrids are true parents of interbreeding. Obtaining and preliminary identification of distant hybrids provided bridge germplasm for promoting gene exchange between radish and Brassica, and provided the basic material for further creation of new germplasm of radish or Brassica crops.