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目的:研究长链非编码RNA SNHG5在胃癌中的表达、功能及机制。方法:实时荧光定量PCR检测SNHG5在胃癌和癌旁组织中的表达差异;在人胃癌细胞中研究过表达或敲低SNHG5对细胞增殖、迁移及侵袭能力的影响;建立裸鼠皮下及转移瘤模型,研究SNHG5在体内对肿瘤发生发展及转移的影响;利用RNA-pulldown联合质谱的策略寻找SNHG5的相互结合蛋白,
Objective: To study the expression, function and mechanism of long-chain non-coding RNA SNHG5 in gastric cancer. Methods: The expression of SNHG5 was detected by real-time fluorescence quantitative PCR in gastric cancer and its adjacent tissues. The effects of over-expression or knockdown of SNHG5 on the proliferation, migration and invasion of human gastric cancer cells were studied. The model of subcutaneous and metastatic tumor was established , To study the effect of SNHG5 on tumorigenesis and metastasis in vivo; to find out the mutual binding protein of SNHG5 by the strategy of RNA-pulldown combined with mass spectrometry,