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以水稻野败型雄性不育系珍汕97A及其保持系珍汕97B的一叶一心期幼苗为试验材料,用对珍汕97A专化致病的稻瘟病菌90-2菌株产生的粗毒素处理幼苗,测定两种试材不同时间叶片中几种细胞保护酶、与活性氧代谢有关的酶、丙二醛含量和可溶性蛋白质含量的动态变化,以探讨A、B两系对专化性毒素的反应差异,进而分析由于细胞质不同而引起病理反应的差异。结果表明:(1)稻瘟病菌90-2毒素处理对珍汕97A的损害大于珍汕97B。(2)引起植株体内与活性氧代谢有关的酶活性、抗性相关酶、丙二醛含量和可溶性蛋白质含量发生变化,这种变化在珍汕97A、B之间有差异;毒素对珍汕97A的过氧化氢酶、过氧化物酶活性有专化性刺激作用。(3)超氧化物歧化酶、苯丙氨酸解氨酶活性上升幅度与珍汕97A、B对稻瘟病菌90-2菌株的抗性大小成正相关,丙二醛含量与抗性大小成负相关。试验结果还表明,粗毒素对幼苗某些蛋白质的表达合成有刺激作用。
Taking one-leaf-one-seedling of the wild-type male sterile line Zhenshan 97A and its maintainer Zhenshan 97B as test materials, the crude toxins produced by strain 90-2 of pathogenic Magnaporthe grisea 97F The seedlings were treated and the dynamic changes of several cytoprotective enzymes, active oxygen metabolism-related enzymes, malondialdehyde content and soluble protein content in leaves of the two test materials at different times were determined to investigate the effects of A and B Of the differences in response, and then analyzed due to different cytoplasmic pathological differences caused by the reaction. The results showed that: (1) 90-2 toxin of Magnaporthe grisea was more harmful to Zhenshan 97A than Zhenshan 97B. (2) The activities of enzymes related to reactive oxygen metabolism in plants were induced, and the contents of resistance-related enzymes, malondialdehyde and soluble protein were changed. The changes were different in Zhenshan 97A and B; Catalase, peroxidase activity has a special stimulus. (3) The increase of superoxide dismutase and phenylalanine ammonia lyase activity was positively correlated with the resistance of Zhenshan 97A and B to Magnaporthe grisea 90-2, and the content of MDA and the resistance were negative Related. The results also show that crude toxin can stimulate the synthesis and expression of certain proteins in seedlings.