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为探讨硫对大葱(Allium fistulosum L.var.giganteam Makino)生长及氮硫吸收同化的影响,采用盆栽沙培方法,研究了0.01、2.01、4.01和6.01 mmol·L-1不同供硫水平下‘天光’大葱的生长量、植株氮硫含量及氮硫同化关键酶活性的变化。结果表明,适当提高供硫水平,可显著促进大葱生长,提高植株氮、硫含量及产量和品质,硫水平过高,则大葱产量降低,品质下降。大葱叶片硫酸化酶(ATPS)活性随硫水平的升高而降低,而乙酰丝氨酸水解酶(OASS)、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)活性在不同生长期的表现不尽相同,大葱生长前期,较高硫水平提高了NR活性,但对GS和OASS活性无显著影响;大葱生长中后期,适量提高供硫水平显著增强了OASS、GS活性,但降低了NR活性,表明供硫水平可显著影响大葱对氮硫的同化利用。综合分析表明,大葱营养液硫水平以2.01 mmol·L-1较佳,其产量较对照0.01 mmol·L-1增加19.13%,且综合品质显著改善。
In order to investigate the effects of sulfur on the growth and nitrogen and sulfur absorption and assimilation of Allium fistulosum L.var.giganteam Makino, The growth of Welsh onion, the content of nitrogen and sulfur in plant and the key enzyme activity of nitrogen and sulfur assimilation. The results showed that proper raising of sulfur supply could significantly promote the growth of green onions and increase nitrogen and sulfur content and yield and quality of the plants. When the sulfur level was too high, the yield and quality of green onions decreased. The activities of sulfatase (ATPS) in Onion leaves decreased with the increase of sulfur level, while the activities of OASS, NR and GS in different growth stages were not At the early stage of onion growth, higher sulfur levels increased NR activity, but had no significant effect on GS and OASS activities. In middle and late growth stages of Welsh onion, moderate levels of sulfur supply significantly enhanced OASS and GS activities but decreased NR activity, It showed that the sulfur supply could significantly affect the assimilation of nitrogen and sulfur in green onions. Comprehensive analysis showed that the nutrient solution sulfur content of Welsh onion was better than 2.01 mmol·L-1, and its yield was increased by 19.13% compared with the control 0.01 mmol·L-1, and the comprehensive quality was significantly improved.