【摘 要】
：
The coloration and hatchability of insect eggs can affect individual and popula-tion survival.However,few genetic loci have been documented to affect both traits,and the genes involved in regulating these two traits are unclear.The silkworm recessive muta
【机 构】
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State Key Laboratory of Silkworm Genome Biology,Key Laboratory of Sericultural Biology and Genetic B
论文部分内容阅读
The coloration and hatchability of insect eggs can affect individual and popula-tion survival.However,few genetic loci have been documented to affect both traits,and the genes involved in regulating these two traits are unclear.The silkworm recessive mutant rel shows both red egg color and embryo mortality.We studied the molecular basis of the rel phenotype formation.Through genetic analysis,gene screening and sequencing,we found that two closely linked genes,BGIBMGA003497 (Bm-re) and BGIBMGA003697 (Bm-Sema1a),control egg color and embryo mortality,respectively.Six base pairs of the Bm-re gene are deleted in its open reading frame,and BmSema1a is expressed at abnormally low levels in mutant rel.BmSemala gene function verification was performed using RNA interference and clustered randomly interspersed palindromic repeats (CRISPR)/CRISPR-associate protein 9.Deficiency of the BmSema1a gene can cause the death of silkworm embryos.This study revealed the molecular basis of silkworm rel mutant formation and indicated that the Sema1a gene is essential for insect embryo development.
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