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目的探讨诱导痰中转化生长因子-β(TGF-β)含量及其作为矽肺早期生物标志物的意义和接尘量对其的影响。方法 160名金矿工人,分为病例组80名(Ⅰ和Ⅱ期矽肺各40例)和对照组80名(接尘但未患矽肺),对照组按接尘量不同又分为4个亚组。,根据安全环境保护部的资料计算出每人的累积总粉尘接触量(CTE)并应用痰液诱导技术取患者的诱导痰并应用酶联免疫吸附试验(ELISA)法测定TGF-β含量。结果病例组TGF-β含量显著高于对照组(t=9.885,P<0.01),病例组中Ⅰ期和Ⅱ期矽肺患者及对照组中按接尘量分组的CTE含量均有统计学意义(F=13.81,P<0.01)。随CTE增加,TGF-β含量随之增加,差异有统计学意义(F=76.0,P<0.01)。结论矽尘所致的纤维化早期TGF-β含量变化规律明显,在纤维化进程中,接尘量对TGF-β含量变化也有显著性影响。
Objective To investigate the content of TGF-β in induced sputum and its significance as an early biomarker of silicosis and its influence on the amount of dust exposure. Methods A total of 160 gold miners were divided into 80 cases (40 cases of stage I and II silicosis) and 80 cases of control group (but not silicosis). The control group was divided into 4 sub-groups group. , Calculate the cumulative total dust exposure (CTE) per person according to the data of the Ministry of Environmental Protection and the sputum induction technique to take the induced sputum of the patients and determine the content of TGF-β by enzyme-linked immunosorbent assay (ELISA). Results The levels of TGF-β in the cases were significantly higher than those in the control group (t = 9.885, P <0.01). The CTE levels in the patients with stage I and II silicosis and those in the control group were all statistically significant F = 13.81, P <0.01). With the increase of CTE, the content of TGF-β increased, the difference was statistically significant (F = 76.0, P <0.01). Conclusion The changes of TGF-β content in the early stages of fibrosis induced by silica dust are obvious. In the course of fibrosis, the dust-holding amount also has a significant effect on the content of TGF-β.