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在最近几十年里,关于抗生素生物作用出现了种种推测,其中一些已被实验结果所证实,而另一些纯粹是一种假说。我们开始研究链霉素生物作用时,发现了该菌细胞壁合成和抗生素合成之间的相互作用,细胞壁合成的许多抑制剂会增加链霉素的产量。由于细胞壁及链霉素均明显含有糖类,我们假定两种生物合成途径都存在一种共同前体,为研究这种共同前体而分离出一种具有抗生素活性的未知结构化合物(X)。本文将讨论链霉素和Park核苷酸共价复合物的分离及化学结构。肽葡聚糖的组成灰色链霉菌菌丝体(24小时菌龄)用超声粉碎,差速离心分离。十二烷基硫酸钠洗涤以除去非肤葡聚糖组份。24小时酸水解,氨基酸分析发现肽葡聚糖含有二氨基庚二酸、丙氨酸、甘氨酸和谷氨酸,其克分子比为
In recent decades there have been speculations about the biological role of antibiotics, some of which have been corroborated by experimental results, while others are purely hypothetical. When we started studying the biological role of streptomycin, we found that there was an interaction between the bacterial cell wall synthesis and antibiotic synthesis and that many inhibitors of cell wall synthesis increased streptomycin production. Since both cell wall and streptomycin apparently contain sugars, we assume that there is a common precursor for both biosynthetic pathways and that an unknown structural compound (X) with antibiotic activity is isolated in order to study this common precursor. This article will discuss the separation and chemical structure of streptomycin and Park nucleotide covalent complexes. Composition of peptidoglycan Streptomyces griseus mycobacterium (24-hour old) was sonicated and differentially separated by centrifugation. Sodium lauryl sulfate was washed to remove the non-dextran components. 24 hours acid hydrolysis, amino acid analysis found that peptidoglycan contains diaminopimelic acid, alanine, glycine and glutamic acid, the molar ratio of