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对15只家兔单侧损毁大脑皮质额顶叶广泛区域,动物存活1～10天不等,心脏灌流处死动物,取延髓和脊髓颈段,固定1周后作各段30μm厚的冰冻连续横切片。切片分为3组,分别用铜银法、Fink-HeimerⅡ法和Fink-Hei-merⅡ后漂白复染法顺行追踪锥体束在脊髓的投射。在光镜下观察,用绘图仪描记出溃变纤维和溃变终末。实验结果表明,密集的溃变纤维显现在皮质损毁同侧的锥体内。在延髓和脊髓的交界处,溃变纤维向背内侧越边交叉行至脊髓灰质后角内。在脊髓节段,溃变纤维仅见于C_1～C_5,并以众多分散小束显现于对侧灰质后角内。位于C_1节段的溃变纤维密集,主要分布在灰质Ⅲ～Ⅴ层。在C_2～C_5节段,溃变纤维逐渐减少,其分布由Ⅲ～Ⅴ层逐渐限定在Ⅴ层的外侧部。溃变终末以C_1、C_2节段为明显,主要分布于灰质Ⅲ～Ⅴ层。对结果的讨论提示,哺乳动物锥体束的行程反映了中枢神经系某种进化关系。为探讨锥体束的生理功能提供了进一步的形态学基础资料。
Fifteen rabbits were unilateral damaged cortical frontal lobe extensive area, the animals survive from 1 to 10 days, cardiac perfusion animals were sacrificed, take the medulla oblongata and the cervical spine, fixed for 1 week after each 30μm thick frozen continuous horizontal slice. The slices were divided into 3 groups, and the cone beam was traced to the spinal cord by Fink-HeimerⅡmethod and Fink-Hei-merⅡafter bleaching and duplicating method respectively. Observed under a light microscope, using a plotter to delineate the degenerative fibers and ulcerated terminals. The experimental results show that the dense degenerated fibers appear in the same side of the cortex damaged cones. At the junction of the medulla oblongata and the spinal cord, the degenerated fibers cross the line to the dorsal angle of the dorsal horn. In the spinal cord segment, the degenerative fibers only found in C_1-C_5 and appeared in the dorsal horn of the contralateral gray matter with many scattered small bundles. The degenerated fibers located in the C_1 segment were dense and mainly distributed in the layers Ⅲ ~ Ⅴ of gray matter. In the C_2 ~ C_5 segment, the degenerated fibers gradually decreased, and their distribution gradually restricted from the Ⅲ ~ Ⅴ layer to the lateral part of the Ⅴ layer. The terminal degeneration of C_1, C_2 segment is obvious, mainly distributed in gray matter Ⅲ ~ Ⅴ layer. The discussion of the results suggests that the mammalian pyramidal tract stroke reflects some evolutionary relationship in the central nervous system. In order to explore the physiological function of pyramidal tract provides further morphological information.