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目的 研究聚腺苷二磷酸核糖聚合酶-１（ＰＡＲＰ-１）在子宫腺肌病在位内膜及异位内膜组织中的表达，讨论ＰＡＲＰ-１与子宫腺肌病上皮间质转化（ＥＭＴ）标志物在子宫腺肌病在位内膜及异位内膜中表达的相关关系。方法 采用免疫组织化学法检测 ＰＡＲＰ-１和 ＥＭＴ标志物包括 Ｅ-钙黏蛋白（Ｅ-ｃａｄｈｅｒｉｎ）、波形蛋白（ｖｉｍｅｎｔｉｎ）、转录调控因子 Ｓｎａｉｌ在子宫腺肌病在位、异位内膜和正常子宫在位内膜组织中的表达，并对 ＰＡＲＰ-１、Ｅ-ｃａｄｈｅｒｉｎ、ｖｉｍｅｎｔｉｎ及 Ｓｎａｉｌ的表达水平进行 Ｓｐｅａｒｍａｎ等级相关性分析。结果 ＰＡＲＰ-１、ｖｉｍｅｎｔｉｎ及 Ｓｎａｉｌ在子宫腺肌病在位及异位内膜中的表达比正常子宫在位内膜表达水平高（Ｐ均 ＜０．０５），Ｅ-ｃａｄｈｅｒｉｎ在子宫腺肌病在位、异位内膜组织中的表达比正常子宫在位内膜组织表达水平低（Ｐ均 ＜０．０５）。在子宫腺肌病在位、异位内膜组织中，ＰＡＲＰ-１的表达水平均与 Ｅｃａｄｈｅｒｉｎ的表达呈负相关，与 ｖｉｍｅｎｔｉｎ及 Ｓｎａｉｌ的表达呈正相关。结论 ＰＡＲＰ-１在子宫腺肌病在位、异位内膜组织中表达增高并与 ＥＭＴ标志物有相关关系，ＰＡＲＰ-１可能参与了子宫腺肌病的 ＥＭＴ过程。
Objective To investigate the expression of PARP-1 in eutopic endometrium and ectopic endometrium of adenomyosis and to discuss the relationship between PARP-1 and adenomyosis EMT) markers in eutopic endometrium and ectopic endometrium of adenomyosis. Methods Immunohistochemical method was used to detect PARP-1 and EMT markers including E-cadherin, vimentin, transcription factor Snail in eutopic and ectopic endometrium and normal The expression of PARP-1, E-cadherin, vimentin and Snail were analyzed by Spearman rank correlation. Results The expressions of PARP-1, vimentin and Snail in eutopic and ectopic endometrium of adenomyosis were higher than those in eutopic endometrium (all P <0.05). The expression of E-cadherin in adenomyosis In eutopic and ectopic endometrial tissues, the expression level of eutopic endometrium was lower than that in normal uterine tissues (all P <0.05). In adenomyosis eutopic and ectopic endometrial tissues, PARP-1 expression levels were negatively correlated with E-cadherin expression, and positively correlated with vimentin and Snail expression. Conclusions The expression of PARP-1 in eutopic and ectopic endometrial tissues of adenomyosis is increased and correlated with EMT markers. PARP-1 may be involved in the process of EMT in adenomyosis.