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目的 验证激光共聚焦显微镜 (CL SM)可否作为研究微球结构的工具 ,并用以获取微球内部结构的信息。方法 微球用复凝聚法制备 ,牛血清白蛋白 (BSA)为模型蛋白药物和聚合物 (明胶与阿拉伯胶 )为成球材料 ,在制备微球前均共价标记了荧光物。应用 CL SM将微球在不同荧光通道下呈像 ,并将微球切割成一系列平行切面分别成像 ,对微球进行三维重建和图像分析。结果 在 CL SM下微球中 BSA与聚合物均呈均匀分布 ,加入BSA不影响聚合物的分布。结论 CL SM可观察载药微球的内部结构 ,而不需事先破坏样品即可获得被包裹药物的定位和成球材料所构成微球的结构资料。
Objective To verify whether laser scanning confocal microscopy (CLSM) can be used as a tool to study the structure of microspheres and obtain the information about the microstructure of microspheres. Methods The microspheres were prepared by complex coacervation. Bovine serum albumin (BSA) was used as the model protein drug and polymer (gelatin and gum arabic) as the material of the spheres. Both of them were covalently labeled with fluorescein before the microspheres were prepared. The microspheres were imaged under different fluorescence channels with CL SM. The microspheres were cut into a series of parallel sections and then imaged respectively. The microspheres were reconstructed and image analyzed. Results The microspheres in CL SM showed uniform distribution of BSA and polymer, and the addition of BSA did not affect the distribution of polymer. Conclusion CL SM can observe the internal structure of drug-loaded microspheres without the need to destroy the sample in advance to obtain the structure of the encapsulated drug and the microsphere structure of the material.