碱茅幼穗在附加有２４－Ｄ１５ｍｇ／Ｌ的ＭＳ培养基中，暗培养条件下两周时形成０５ｃｍ２大小的愈伤组织块，其发生率为４３％。培养３周后形成旺盛生长的肉黄色愈伤组织。愈伤组织的分化是在含ＫＴ１０ｍｇ／Ｌ、ＮＡＡ０５ｍｇ／Ｌ的ＭＳ培养基上２～４周时出现绿色芽点，继续培养１～２周即可得到分化植株，将该植株移至不含激素的ＭＳ或Ｎ６培养基上时，光照条件下形成绿色分化植株。 In the medium with 24-D15mg / L added MS medium, the callus of P. arborescens formed 05cm2 size callus in two weeks under dark culture condition, the incidence rate was 43%. After 3 weeks of cultivation, flesh-yellow callus grew vigorously. The differentiation of callus is that green shoots emerge at 2-4 weeks on MS medium containing KT10mg / L and NAA05mg / L, and differentiated plants can be obtained by further culturing for 1 ~ 2 weeks. To hormone-free MS or N6 medium, green differentiated plants formed under light conditions.