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Objective To investigate expression and localization of Calbindin-d28k (CaBP-d28k) in human ovary.Methods The expression of the CaBP-d28k protein and gene in 23 human ovarian samples was measured by immunohistochemistry and reverse transcription polymerase chain reaction (RTPCR) methods.Results Positive expression of the CaBP-d28k protein and mRNA was observed in human ovary.The ovarian expression of the CaBP-d28k protein appeared in the cytoplasm of oocytes,granulose cells,theca cells,and two types of cells of corpus luteum.From primordial follicle,primary follicle to secondary follicle,increased CaBPd28k levels were showed (P<0.05),and the CaBP-d28k expression in secondary follicle had the highest level.Conclusion Human ovarian tissue locally expresses CaBP-d28k,and its expression level increases with follicular development.
Objective To investigate expression and localization of Calbindin-d28k (CaBP-d28k) in human ovary. Methods The expression of the CaBP-d28k protein and gene in 23 human ovarian samples was measured by immunohistochemistry and reverse transcription polymerase chain reaction (RTPCR) methods. Results Positive expression of the CaBP-d28k protein and mRNA was observed in human ovary.The ovarian expression of the CaBP-d28k protein was in the cytoplasm of oocytes, granulose cells, theca cells, and two types of cells of corpus luteum. follicle, primary follicle to secondary follicle, increased CaBPd28k levels were showed (P <0.05), and the CaBP-d28k expression in secondary follicle had the highest level. Conclusions Human ovarian tissue locally expresses CaBP-d28k, and its expression level increases with follicular development.