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目的:对暗紫贝母愈伤组织和不定芽诱导进行研究。方法:以暗紫贝母新鲜鳞茎为外植体,在附加NAA和6-BA不同浓度配比的MS培养基上进行离体培养。结果:鲜鳞茎能诱导形成愈伤组织和不定芽。其中,NAA1.2mg/L+6-BA1.8mg/L的MS培养基中外植体愈伤组织诱导率最高,达53.3%;NAA1.2mg/L+6-BA1.6mg/L的MS培养基中外植体不定芽诱导率最高,达73.3%。结论:建立了暗紫贝母愈伤组织和不定芽诱导的培养方法。
Objective: To study the induction of callus and adventitious buds of Fritillary. Methods: Fresh bulbulemus bulb was used as explants and cultured on MS medium supplemented with different concentrations of NAA and 6-BA. Results: Bulblets can induce the formation of callus and adventitious buds. Among them, the explants callus induction rate was the highest in NAA1.2mg / L + 6-BA1.8mg / L MS medium, reaching 53.3%; NAA1.2mg / L + 6-BA1.6mg / L MS medium The highest induction rate of adventitious buds in explants was 73.3%. Conclusion: The method of culturing callus and adventitious buds of Fritillaria ussuriensis was established.