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目的:建立大鼠脊髓半横断伤联合架桥模型,为研究脊髓损伤提供动物模型。方法:制作大鼠脊髓半横断伤模型,然后取大鼠前肢正中神经,并于半横断伤两端行正中神经架桥术。术后4周,左心室灌注固定取材,免疫组化染色检测GFAP、RECA、NF-200;另一部分动物行单宁酸-氯化铁灌注;观察移植物内有无血管、血管内有无血流、血管与周边神经纤维的关系。结果:外周神经架桥后4周,移植正中神经贴合于脊髓背侧1/2。移植神经内有RECA阳性的血管存在,而且有血流可以到达移植物内部,且神经纤维(NF-200阳性)与星形胶质细胞(GFAP阳性)关系紧密。结论:大鼠脊髓半横断伤联合正中神经架桥术后,由宿主可以向移植物内长入新生血管,血管有利于神经纤维的存活及生长。本模型为较好的外周神经移植的存活模型,可为进一步的深入研究提供一定的依据。
OBJECTIVE: To establish a rat model of spinal cord hemisection combined with bridging and to provide an animal model for the study of spinal cord injury. Methods: The rat model of spinal cord transection was made, then the median nerve of the forelimb was taken and the median neurosurgery was performed at both ends of the half-transected injury. Four weeks after operation, the left ventricular perfusion was fixed and immunohistochemistry was used to detect GFAP, RECA and NF-200. The other animals were given tannic acid-ferric chloride infusion. Flow, blood vessels and peripheral nerve fibers. Results: Four weeks after the peripheral nerve grafts, the median nerve was grafted to the dorsal 1/2 of the spinal cord. RECA positive blood vessels exist within the graft nerve, and blood flow can reach the inside of the graft, and nerve fibers (NF-200 positive) and astrocytes (GFAP positive) are closely related. CONCLUSIONS: After the rats have been transected by transection of the spinal cord and the median nerve bridge, the host can grow new blood vessels into the graft. The blood vessels are conducive to the survival and growth of nerve fibers. This model is a good survival model of peripheral nerve graft, which can provide some evidence for further research.