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目的 探讨PKC不同亚型在原代培养脊髓神经元突起生长中的作用。 方法 采用免疫组织化学技术研究蛋白激酶C(PKC) 4种亚型 (α、βⅠ、βⅡ和ε)在胎鼠脊髓原代培养神经元突起生长过程中的表达与分布 ,借助计算机图像分析系统同步测定突起长度和胞体面积。 结果 原代培养神经元生长过程中 ,7d前随着PKC 4种亚型的表达逐渐增加 ,神经元胞体逐渐长大及突起逐渐延长 ;7d以后无论在胞体还是突起中 ,PKC 4种亚型的表达均下降 ,突起开始萎缩 ,胞体内出现空泡。相关分析发现 βⅡ亚型与突起生长关系最密切 (r =0 73,P <0 0 1)。 结论 PKC可能参与了神经元的生长过程 ;βⅡ型PKC在培养脊髓神经元突起生长中的作用较为重要
Objective To investigate the role of different subtypes of PKC in the process of primary cultured spinal cord neurons. Methods Immunohistochemistry was used to study the expression and distribution of four subtypes of PKC (α, βⅠ, βⅡ and ε) during the process of neurite outgrowth in embryonic primary cultured spinal cord. With computer image analysis system synchronization Protrusion length and cell body area were measured. Results In primary cultured neurons, the expression of 4 subtypes of PKC gradually increased before 7 days, and the somatic cell bodies of neurons gradually grew up and the protuberances gradually grew. In the cytoplasm or protuberances after 7 days, the four subtypes of PKC The expression decreased, the process of atrophy began to appear vacuoles in the cell body. Correlation analysis showed that βⅡ subtype had the closest relationship with the process of protrusion (r = 0 73, P <0.01). Conclusions PKC may be involved in the process of neuron growth. The effect of β-type PKC on the process of neurite outgrowth in cultured spinal cord is more important