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目的观察布拉氏酵母菌对葡聚糖硫酸钠(dextran sulfacte sodium,DSS)诱导的实验性结肠炎小鼠肠黏膜屏障的影响。方法将50只BALB/c小鼠随机分成5组,分别为:正常对照组(A)、模型组(B)、美沙拉嗪组(C)、布拉氏酵母菌组(D)、联合治疗组(E)。采用2.5%DSS溶液诱导小鼠实验性结肠炎动物模型,将C、D、E组小鼠分别给予美沙拉嗪、布拉氏酵母菌、美沙拉嗪加布拉氏酵母菌联合灌胃治疗,计算小鼠疾病活动指数(disease activity index,DAI)评分,评估结肠黏膜组织损伤程度,并用Western blot法检测结肠黏膜紧密连接蛋白ZO-1、Occludin表达水平。结果模型组小鼠的DAI评分及结肠黏膜组织损伤程度明显高于正常组(p<0.05),ZO-1及Ocluddin表达水平明显低于正常组(p<0.05)。与模型组相比,美沙拉嗪组、布拉氏酵母菌组、联合治疗组的DAI评分及结肠黏膜组织损伤程度均明显减低(p<0.05),ZO-1及Ocluddin表达水平明显增多(p<0.05),联合治疗组效果更好。结论布拉氏酵母菌可对DSS诱导的结肠炎肠黏膜屏障功能起到保护作用,与美沙拉嗪联合应用效果更佳。
Objective To observe the effect of Saccharomyces boulardii on the intestinal mucosal barrier of mice with experimental colitis induced by dextran sulfacte sodium (DSS). Methods Fifty BALB / c mice were randomly divided into five groups: normal control group (A), model group (B), mesalazine group (C), Braga group (D) Group (E). The mice model of experimental colitis was induced by 2.5% DSS solution. The mice in groups C, D and E were treated with mesalazine, Saccharomyces boulardii, Mesalazine and Gaborala saccharification respectively, The mouse disease activity index (DAI) score was calculated to evaluate the degree of colonic mucosal injury. Western blot was used to detect the expression of ZO-1 and Occludin in colonic mucosa. Results The DAI score and damage of colonic mucosa in model group were significantly higher than those in normal group (p <0.05). The expression of ZO-1 and Ocluddin in model group was significantly lower than that in normal group (p <0.05). Compared with the model group, the DAI scores and the damage degree of colonic mucosa of mesalazine group, Brays yeast group and combination therapy group were significantly decreased (p <0.05), and the expression levels of ZO-1 and Ocluddin were significantly increased (p <0.05), combined treatment group is better. Conclusion B. Saccharomyces can protect the intestinal mucosal barrier function induced by DSS, which is more effective when combined with mesalazine.