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利用RAPD-PCR及ISSR-PCR两种分子标记技术,分析了七里海中华绒螯蟹第6代繁育群体的遗传多样性。用10个多态性高的RAPD引物对24个七里海中华绒螯蟹样本个体进行扩增,共检测出107个不同的扩增位点,扩增片段大小为300~2300bp。其中多态位点总数为66个,平均多态位点比例为61.32%,群体内香农-维纳多样性值为0.1714。用8个多态性高的ISSR引物对24个个体共检测到106个位点,扩增片段为200~1500bp。其中多态性位点79个,平均多态位点百分率为74.53%,群体内香农-维纳多样性值为0.1778。结果显示,该群体的多态位点比例和香农-维纳多样性值均较大,说明其有较丰富的遗传多样性,同时也说明RAPD和ISSR技术用于七里海中华绒螯蟹核基因组的遗传多样性分析,具有较高的检出率和灵敏度。
Using RAPD-PCR and ISSR-PCR, we analyzed the genetic diversity of 6th-generation Chinese mitten crab (Eriocheir sinensis) breeding population. Twenty-seven individuals of Eriocheir sinensis were amplified using 10 RAPD primers with high polymorphism. A total of 107 different amplification sites were detected, and the size of the amplified fragment was 300-2300 bp. The total number of polymorphic loci was 66, the average percentage of polymorphic loci was 61.32%, and Shannon-Wiener diversity in population was 0.1714. A total of 106 sites were detected in 24 individuals using 8 ISSR primers with high polymorphism. The amplified fragment ranged from 200 to 1500 bp. Among them, 79 were polymorphic and the average percentage of polymorphic loci was 74.53%. The Shannon-Wiener diversity in the population was 0.1778. The results showed that the population of polymorphic loci and Shannon-Wiener diversity values were large, indicating that they have a rich genetic diversity, but also shows that RAPD and ISSR technology used in the Qilihai Eriocheir sinensis nuclear genome Genetic diversity analysis, with a high detection rate and sensitivity.