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目的比较巴马小型猪诱导型肝硬化造模前(正常猪)和造模成功后(肝硬化猪)肠道乳杆菌的变化情况,探讨小型猪肝硬化模型在肝硬化肠道微生态研究中的适用性。方法收集肝硬化造模前和CCl4诱导肝硬化造模成功后巴马小型猪的新鲜粪便,提取粪便总菌DNA,用乳杆菌特异性引物进行PCR扩增,对扩增产物进行变性梯度凝胶电泳(Denaturing Gradient Gel-Electrophoresis,DGGE),即用PCR-DGGE分析巴马小型猪肝硬化前后肠道乳杆菌的相似性和多样性。结果聚类分析和主成分分析显示巴马小型猪肝硬化前(正常猪)和肝硬化后混杂排列,无明显界限;多样性数据分析显示巴马小型猪肝硬化前后肠道乳杆菌的丰富度(S)、微生物区系Shannon-Wiener指数(H′)和均匀度(E)差异均无统计学意义(P>0.05)。结论巴马小型猪肝硬化后肠道乳杆菌与正常猪比较差异无统计学意义。
Objective To compare the changes of lactobacilli in intestinal mucosa of Bama miniature pig before induced cirrhosis (normal pig) and after successful modeling (cirrhosis pig), and to investigate the effect of miniature pig liver cirrhosis Applicability. Methods The fresh faeces of Bama miniature pig before cirrhosis modeling and CCl4 induced cirrhosis modeling were collected. The DNA of the total faecal bacteria was extracted and amplified by PCR using Lactobacillus specific primers. The amplified products were subjected to denaturing gradient gel Denaturing Gradient Gel-Electrophoresis (DGGE) was used to analyze the similarity and diversity of Lactobacillus entericum before and after cirrhosis of Bama mini-pigs by PCR-DGGE. Results Cluster analysis and principal component analysis showed that there was no obvious boundary between the Bama mini-pig before cirrhosis (normal pig) and cirrhosis, and the diversity data showed that the richness of intestinal lactobacilli before and after Bama mini-pig cirrhosis (S), Shannon-Wiener index (H ’) and evenness (E) of microbial flora were not significantly different (P> 0.05). Conclusion There is no significant difference between Lactobacillus of Bama miniature pigs after liver cirrhosis and normal pigs.