论文部分内容阅读
[目的]探索建立稳定的小鼠视神经损伤模型实验方法。[方法]选用成年C57BL/6小鼠60只,手术进行左眼视神经钳夹损伤,右眼设为正常对照眼,随机分为5个组,每组12只,分别在损伤后1d、3d、5d、7d、14d5个时间点摘除眼球。取眼球和视神经行冰冻切片,HE染色,观察组织形态并对神经胶质细胞计数。[结果]光镜下观察损伤部位形成损伤区域,损伤区域组织染色浅,未损伤眼视神经纤维排列整齐,胶质细胞与神经纤维走向一致,呈极性排列。损伤眼视神经纤维排列紊乱,胶质细胞杂乱无序。损伤后视神经胶质细胞计数减少,少于未损伤侧视神经,两者具有统计学差异。[结论]成功建立小鼠神经损伤模型,视神经损伤后形态学有显著改变。
[Objective] To explore a stable experimental model of optic nerve injury in mice. [Methods] Sixty adult C57BL / 6 mice were selected and the left optic nerve was harmed by surgery. The right eye was set as the normal control eye and randomly divided into 5 groups of 12 rats, 5d, 7d, 14d5 time points removed the eye. The eye and optic nerve were frozen and stained with HE. The morphology and glial cell count were observed. [Result] The damaged area was observed under the light microscope to form the damaged area. The damaged area was stained lightly, the fibers of the optic nerve fibers were arranged neatly, the glial cells and the nerve fibers were in the same direction and arranged in a polar order. Injured optic nerve fiber disorder, glial disorder disorder. After injury, the number of glial cells decreased, less than the uninjured lateral optic nerve, both with statistical significance. [Conclusion] The model of neural injury in mice was established successfully and the morphological changes of optic nerve were significantly changed.