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目的 研究氟中毒对大鼠肝、肾组织磷脂脂肪酸组成的影响。方法 选用 Wistar纯系大鼠 ,饲以高浓度含氟水 1个月 ,来复制慢性氟中毒动物模型。用改良 Falch方法提取和分离动物肝、肾组织磷脂 ,用气相色谱法分离和测定磷脂各类脂肪酸构成。结果 所有饲以高浓度氟水的动物均有不同程度的氟斑牙形成 ,尿氟含量增高 ,肝和肾组织蛋白质含量降低等慢性氟中毒的表现 ;氟中毒动物肝、肾组织磷脂脂肪酸组成发生异常改变 ,表现为多不饱和脂肪酸减少 ,饱和脂肪酸组成增加 ,减少的多不饱和脂肪酸种类是花生四稀酸和二十二碳六烯酸。结论 表明长期摄入过量氟可导致肝、肾组织磷脂脂肪酸组成改变 ,磷脂多不饱和脂肪酸减少可能与自由基水平升高所造成的脂质过氧化增强有关
Objective To study the effects of fluorosis on the phospholipid fatty acid composition in rat liver and kidney. Methods Wistar rats were fed with high concentration of fluoride water for one month to copy chronic fluorosis animal model. The modified Falch method was used to extract and separate animal liver and kidney tissue phospholipids. The fatty acids were separated and determined by gas chromatography. Results All animals fed with high concentration of fluoride water had different levels of dental fluorosis formation, increased urinary fluoride content and decreased protein content in liver and kidney tissues. The composition of phospholipid fatty acids in liver and kidney tissue of fluorosis animals Abnormal changes, manifested as polyunsaturated fatty acids decreased, saturated fatty acid composition increased, decreased polyunsaturated fatty acids are arachidonic acid and docosahexaenoic acid. Conclusion: Long-term intake of fluoride can lead to liver and kidney phospholipid fatty acid composition changes, decreased phospholipid PUFAs may be associated with increased levels of free radicals caused by increased lipid peroxidation