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本研究对2015年分离自福建省龙岩市的139株禽致病性大肠杆菌(Avian pathogenic Escherichia coli,APEC)的血清型、毒力因子分布及耐药性等进行检测,并对血清型与耐药性、毒力因子分布和耐药性之间的相关性进行分析,以期为APEC的防控提供参考。对139株分离菌株的血清型检测结果表明,O1血清型(11株)、O2血清型(14株)和O78血清型(14株)占全部菌株的28%;对分离菌株毒力基因检测结果表明,4%和7.1%的菌株检测到papC基因和vat基因,14.3%和19.3%的菌株检测到aat A基因和cva/cvi基因。而iuc D(82%)、tsh(59%)、iss(55%)和irp2(44.6%)基因在分离菌株中具有较高的检出率;药敏实验结果表明,分离株存在多重耐药性,99%以上分离菌株对红霉素、克林霉素和利福平耐受,86%的分离菌株对10种以上抗生素耐受,57.3%的菌株对13种药物都耐受;对血清型、耐药性与致病性之间的分析结果表明,分离的O1、O2和O78血清型的菌株,其中50%的菌株是含有5种以上毒力基因的强毒株,且在分离的含有5种以上毒力基因的强毒株中,50%的菌株对13种抗生素耐受,90%的菌株对10种以上药物耐受。
In this study, serotype, virulence factor distribution and drug resistance of 139 avian pathogenic Escherichia coli (APEC) strains isolated from Longyan City, Fujian Province in 2015 were tested. The serotype and resistance Drug resistance, virulence factor distribution and drug resistance between the analysis, in order to provide a reference for the prevention and control of APEC. Serotypes of 139 isolates showed that the serotypes of O1 serotypes (11 strains), O2 serotypes (14 strains) and O78 serotypes (14 strains) accounted for 28% of the total isolates. The results of virulence genes of isolates The results showed that papC gene and vat gene were detected in 4% and 7.1% strains, and aat A gene and cva / cvi gene were detected in 14.3% and 19.3% of strains. However, the iuc D (82%), tsh (59%), iss (55%) and irp2 (44.6%) genes had higher detection rates in the isolates. The results of drug sensitivity test showed that the isolates had multiple drug resistance More than 99% isolates were resistant to erythromycin, clindamycin and rifampicin, 86% isolates were resistant to more than 10 antibiotics and 57.3% strains were resistant to 13 drugs; to serum The results of the analysis between type, drug resistance and pathogenicity showed that isolates of O1, O2 and O78 serovars, of which 50% were virulent strains containing more than 5 virulence genes, Among the virulent strains containing more than 5 virulence genes, 50% strains were resistant to 13 antibiotics and 90% strains were resistant to more than 10 drugs.