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以我国闻名于世的珍稀野生果树新疆野扁桃为试材,利用蔷薇科果树自交不亲和性通用引物组合,采用S-RNase等位基因PCR扩增,RT-PCR和RACE等技术方法,克隆了新疆野扁桃不同株系的S-RNase基因,以期为新疆野扁桃自交不亲和分子机理的进一步研究提供基本的资料。结果表明:从新疆野扁桃不同株系中克隆鉴定出了6个新的S-RNase基因,分别命名为PteS_(10)(GeneBank登陆号:KJ755352),PteS_(11)(GeneBank登陆号:KJ755353),PteS_(12)(GeneBank登陆号:KJ755354),PteS_(13)(GeneBank登陆号:KJ755355),PteS_(14)(GeneBank登陆号:KJ755356)和PteS_(15)(GeneBank登陆号:KJ755357);并对这6个新基因进行了序列生物信息学分析,为基于新疆野扁桃自交不亲和特性的遗传改良以及分子调控模式奠定试验基础。
Based on the common primer combinations of Rosaceae fruit tree self-incompatibility, we used the PCR-amplified S-RNase alleles, RT-PCR and RACE techniques to study the genetic diversity of rare wild fruit trees in Xinjiang, The S-RNase gene was cloned from different strains of wild almond trees in Xinjiang in order to provide basic information for further research on the molecular mechanism of self-incompatibility in wild almonds in Xinjiang. The results showed that 6 new S-RNase genes were cloned from different strains of wild Almond, named PteS_ (10) (GeneBank accession number: KJ755352), PteS_ (11) (GeneBank accession number: KJ755353) , PteS_ (12) (GeneBank Accession No. KJ755354), PteS_ (13) (GeneBank Accession No. KJ755355), PteS_ (14) (GeneBank Accession No. KJ755356) and PteS_ (15) (GeneBank Accession No. KJ755357) Sequence bioinformatics analysis of these six new genes was carried out to lay the experimental foundation for genetic improvement and molecular regulation based on self-incompatibility of Wild Almond.