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目的研究(艹猪)苓得糖抗小鼠HepA瘤细胞的可能机制。方法采用免疫组化方法研究(艹猪)苓多糖对小鼠HepA瘤细胞P16、Rb基因及TNF-α表达影响。结果 (艹猪)苓多糖作用后,小鼠HepA瘤细胞P16基因表达蛋白(P16蛋白)表达显著增强(P<0.01);小鼠HepA瘤细胞Rb基因表达蛋白(P105-Rb蛋白)表达增强(P<0.05),小鼠HepA瘤细胞TNF-α表达显著增强(P<0.01)。结论 (艹猪)苓多糖对小鼠HepA瘤细胞P16、Rb基因及TNF-α表达有激活作用。对抑癌基因P16、Rb基因及TNF-α表达的激活可能是(艹猪)苓多糖抗肿瘤的普遍机制之一。
Objective To study the possible mechanism of Lingtang sugar against mouse HepA tumor cells. Methods Immunohistochemical method was used to study the effect of Polysaccharide (Pock) on the expression of P16, Rb gene and TNF-α in mouse HepA tumor cells. Results The expression of P16 gene in HepA tumor cells was significantly increased (P <0.01), and the expression of Rb gene (P105-Rb protein) was increased in HepA tumor cells P <0.05). The expression of TNF-α in HepA tumor cells was significantly increased (P <0.01). CONCLUSION: The polysaccharides of Zizyphus jujubae can activate the expression of P16, Rb gene and TNF-α in mouse HepA tumor cells. The activation of tumor suppressor genes P16, Rb and TNF-α may be one of the most common mechanisms of antitumor activity of Polysaccharides from Schistosoma japonicum.