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目的研究酸性氧化电位水对白色念珠菌杀灭的影响因素。方法采用悬液定量杀菌试验法对酸性氧化电位水杀灭白色念珠菌的影响因素进行了实验室观察。结果在清洁条件下,含有效氯为53.7 mg/L的酸性氧化电位水对白色念珠菌作用3 min或有效氯≥65.5 mg/L作用1 min,其杀灭对数值便可达4.67以上。在有机干扰物的条件下,含有效氯为65.5 mg/L的酸性氧化电位水对白色念珠菌作用1 min,菌液内含3 g/L干扰物组的杀灭对数值为1.31,含30 g/L干扰物组的杀灭对数值仅为0.22。随着pH值的升高和氧化还原电位值的下降,酸性氧化电位水杀菌效果降低。在高ACC时,在pH≤8的范围内,KL都约为5.86,受pH变化的影响不大。结论酸性氧化电位水对白色念珠菌具有很好的杀灭效果,且随有效氯的提高而增强;有机干扰物对酸性氧化电位水的杀菌效果有很强的抑制作用;EOW在酸性条件下的杀菌效果优于碱性条件;EOW的高杀菌效果是以ACC为主导、低pH及高ORP为重要促进的三者协同作用的结果。
Objective To study the factors affecting the killing of Candida albicans by acidic oxidation potential water. Methods Suspension quantitative bactericidal test was used to observe the influencing factors of acid oxidizing potential water to kill Candida albicans. Results Under the cleaning conditions, the acid oxidation potential water containing 53.7 mg / L of available chlorine had the effect of killing Candida albicans for 3 min or the effective chlorine ≥ 65.5 mg / L for 1 min, killing the logarithm value up to 4.67 or above. Under the conditions of organic interferences, the acid oxidation potential water containing 65.5 mg / L of effective chlorine had a 1 min exposure to Candida albicans, and the kill logarithm of 3 g / The kill logarithm for the g / L interferent group was only 0.22. With the increase of pH value and the decrease of redox potential, the bactericidal effect of acid oxidation potential water is reduced. At high ACC, KL was about 5.86 in the range of pH≤8, which was not affected by the pH change. Conclusions Acidic oxidizing potential water has a good killing effect on Candida albicans and increases with the increase of available chlorine. Organic interferences have a strong inhibitory effect on the bactericidal effect of acidic oxidizing potential water. Under the acidic conditions Bactericidal effect is better than alkaline conditions; EOW high bactericidal effect is ACC-led, low pH and high ORP is important to promote the synergistic effect of the three.