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【目的】粒长、粒宽是小麦种子重要的形态性状,该性状对籽粒的外观商品品质、产量及磨粉品质均至关重要,研究不同环境条件下小麦粒长、粒宽的单个标记和复合区间作图的QTL定位,对小麦粒长、粒宽的分子标记辅助选择具有重要参考作用。【方法】应用一个由115个系组成的W7984/Opata 85重组自交系(RIL)群体,建立了由394个DNA分子标记组成的遗传连锁图,在2种不同环境条件下对小麦粒长、粒宽进行了单个标记的回归分析和复合区间作图的QTL定位。【结果】在单个标记的回归分析中检测到5个粒长的QTLs、3个粒宽的QTLs;复合区间作图分析结果表明,控制粒长的QTLs分别位于5BL和7DS上,在5BL上的贡献率为20.20%~20.81%,LOD值为4.50~4.55;在7DS上的贡献率为13.54%~13.91%,LOD值为2.94~3.20。控制粒宽的QTL位于2B上,贡献率为13.71%~19.30%,LOD值为2.98~4.18。【结论】位于5B和7D上的控制粒长的QTL和位于2B上的控制粒宽的QTL在2种条件下均能检测到。
【Objective】 Grain length and grain width are the important morphological traits of wheat seeds. The traits are crucial to the quality, yield and milling quality of wheat seeds. A single marker for grain length and grain width under different environmental conditions QTL mapping of composite interval mapping is of great importance to wheat grain length and grain width in molecular marker-assisted selection. 【Method】 A genetic linkage map consisting of 394 DNA molecular markers was constructed using a W7984 / Opata 85 recombinant inbred line (RIL) population composed of 115 lines. The genetic linkage map was constructed under two different environmental conditions, including grain length, Regression analysis of single marker and QTL mapping of compound interval mapping were carried out for grain width. 【Result】 Five QTLs with grain length and three grain width QTLs were detected in the regression analysis of single marker. The results of the composite interval mapping showed that the QTLs controlling the grain length were located on 5BL and 7DS, respectively, on 5BL The contribution rates ranged from 20.20% to 20.81% and the LOD values ranged from 4.50 to 4.55. The contribution rates at 7DS ranged from 13.54% to 13.91% and the LOD values ranged from 2.94 to 3.20. The QTL controlling the grain width is located on 2B, with the contribution rate of 13.71% to 19.30% and the LOD value of 2.98 to 4.18. 【Conclusion】 The QTL controlling the grain length at 5B and 7D and the QTL controlling grain width at 2B can be detected under both conditions.