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目的:研究腺样囊性癌ACC-2细胞来源的外泌体能否靶向正常唾液腺上皮细胞(SGECs),为进一步研究ACC来源的外泌体在ACC侵袭和转移中的调节作用奠定基础。方法:通过组织块法原代培养人SGECs,应用免疫细胞化学染色对其来源进行鉴定;将ACC-2细胞来源的外泌体用荧光染料PKH67标记后与SGECs共培养,用扫描共聚焦显微镜(LSCM)观察SGECs对ACC-2来源的外泌体的摄取情况。结果:原代培养的SGECs高表达角蛋白CK19,不表达α-MSA,说明其来源可能为腺泡上皮或导管上皮细胞,而非间质和肌上皮细胞。在LSCM下观察到携带PKH67标记的外泌体可以进入SGECs内,主要分布于核周的胞质中。结论:SGECs可摄取ACC-2细胞来源的外泌体,提示可能为ACC来源外泌体的靶细胞之一。
OBJECTIVE: To investigate whether exosomes derived from ACC-2 cells can target normal salivary gland epithelial cells (SGECs) and lay the foundation for the further study on the regulation of ACC-derived exosomes on ACC invasion and metastasis. Methods: Human primary SGECs were cultured by tissue block method and their origin was identified by immunocytochemical staining. ACC-2 cell-derived exosomes were labeled with fluorescent dye PKH67 and co-cultured with SGECs. Scanning confocal microscopy LSCM) to observe the uptake of ACC-2-derived exosomes by SGECs. Results: Primary cultured SGECs highly expressed keratin CK19, but did not express α-MSA, indicating that the origin of SGECs may be acinar epithelial or ductal epithelial cells rather than interstitial and myoepithelial cells. In LSCM, it was observed that exosomes bearing PKH67 marker could enter into SGECs, mainly distributed in the cytoplasm of perinuclear. Conclusion: SGECs can ingest ACC-2-derived exosomes, suggesting that SGECs may be one of the target cells for ACC-derived exosomes.