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目的 :探讨血管内皮生长因子 (VEGF)在卵巢癌浸润转移中的作用。方法 :阳离子脂质体介导VEGF165质粒转染 2株卵巢癌细胞CaOV 3、COC1,并经逆转录聚合酶链式反应 (RT PCR)、Western免疫印迹检测转染VEGF165质粒前后癌细胞VEGFmRNA及其蛋白表达水平 ;用Boyden小室体外侵袭实验比较VEGF165质粒转染前后以及单克隆抗体作用后 2株癌细胞通过人工重组基底膜的能力强弱变化。结果 :VEGF165质粒转染后 ,2株细胞mRNA和蛋白表达水平均较转染前明显增强 (P <0 .0 5 )。VEGF165质粒转染的癌细胞CaOV 3、COC1穿过人工重组基底膜的相对百分率分别为 4 2 .5± 4 .1、2 6 .8± 2 .4 ,明显高于对照组穿膜细胞相对百分率 (2 4 .7± 1.9、8.6± 1.1) (P <0 .0 5 ) ;而在VEGF单克隆抗体存在时 ,2株癌细胞穿膜细胞相对百分率 (10 .2± 0 .7、5 .4± 0 .3)较处理前均有不同程度的下降 (P <0 .0 5 )。结论 :VEGF参与了卵巢癌转移的侵袭、浸润阶段 ,应用VEGF单克隆抗体可针对性地抑制VEGF介导的卵巢癌侵袭、浸润。
Objective: To investigate the role of vascular endothelial growth factor (VEGF) in invasion and metastasis of ovarian cancer. METHODS: Two ovarian cancer cell lines, CaOV 3 and COC1, were transfected with cationic liposome-mediated VEGF165 plasmid and VEGFmRNA and VEGFmRNA were detected by reverse transcriptase polymerase chain reaction (RT PCR) and Western immunoblotting before and after transfection with VEGF165 plasmid Protein expression level; using Boyden chamber in vitro invasion assay VEGF165 plasmid transfection before and after the role of monoclonal antibody by 2 cancer cells by artificial recombinant basement membrane ability to change. Results: After transfected with VEGF165 plasmid, the mRNA and protein expression levels of both cells were significantly increased compared with that before transfection (P <0.05). VEGF165 plasmid transfected cancer cells CaOV 3, COC1 through the artificial recombinant basement membrane relative percentage were 42.5 ± 4 .1,26 .8 ± 2 .4, was significantly higher than the control group, the relative percentage of transmembrane cells (24.7 ± 1.9, 8.6 ± 1.1) (P <0.05), while in the presence of VEGF monoclonal antibody, the relative percentage of transmembrane cells in the two cancer cells was (10.2 ± 0.7) 4 ± 0 .3) than before treatment, there are varying degrees of decline (P <0. 05). Conclusion: VEGF is involved in the invasion and infiltration of ovarian cancer. VEGF monoclonal antibody can specifically inhibit VEGF-mediated invasion and infiltration of ovarian cancer.