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粳稻品种东农415自育成以来一直以其早熟、抗病、高产特性而著称,在黑龙江省稻瘟病高发区种植20多年均表现高抗稻瘟病。本研究利用158个采集于黑龙江省不同稻区的稻瘟病菌株对东农415进行接种鉴定,结果表明东农415对黑龙江省稻瘟病菌株有很强的抗性,抗谱高达89.2%。以东农415与丽江新团黑谷(LTH)杂交衍生的F1和F2群体为遗传分析试验材料,通过接种鉴定,发现东农415对稻瘟病菌株F-10-11的抗性由一个显性基因控制。进一步采用分子标记结合隐性群体分离分析法,以对菌株F-10-11极端感病的99个F2单株为作图群体,将东农415的抗病基因定位在第2染色体,距离基因两侧标记RM5300和RM213的遗传距离分别为7.6和3.0 c M,暂命名为Pi-dn(t)。将Pi-dn(t)位点映射到水稻参考基因组图谱上,在抗病位点基因组区段内发现3个编码基因Os02g56010、Os02g55540和Os02g56400具有抗病基因结构域,可作为Pi-dn(t)的候选基因。
The japonica rice variety Dongnong 415 has been known for its precocious, disease-resistant and high-yielding characteristics since it was bred. It has been shown to be highly resistant to blast in more than 20 years of high-yield rice blast areas in Heilongjiang Province. In this study, 158 were collected from different strains of rice blast in Heilongjiang Province, 415 East farming inoculation, the results show that the East has a strong agricultural 415 strains resistant to rice blast in Heilongjiang province, resistance spectrum up to 89.2%. F1 and F2 populations derived from hybridization between Dongnong 415 and Lijiang Xintiandi Heigu (LTH) were used as genetic analysis materials. Through inoculation identification, the resistance of Dongnong 415 to Magnaporthe grisea F-10-11 was determined by a dominant Gene control. Further use of molecular markers isolated population of implicit binding assay to extreme strains F-10-11 99 susceptible F2 individuals of the mapping population, the positioning Dongnong resistance genes in chromosome 2 of 415, from the gene The genetic distances between markers RM5300 and RM213 on both sides were 7.6 and 3.0 cM, respectively, tentatively named Pi-dn (t). Mapping Pi-dn (t) to the site of the rice genome map reference, three coding genes found Os02g56010, Os02g55540 Os02g56400 and having resistance gene in the resistance domain sites in the genome segments, as Pi-dn (t ) Candidate genes.