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目的:探讨血清总IgE、INF-γ、IL-4作为卵清白蛋白致敏实验性变应性鼻炎(AR)大鼠模型建立客观评价指标及意义。方法:以SD大鼠为实验对象,随机分为AR组和对照组,每组10只。AR组以卵清白蛋白作为致敏原,对照组以生理盐水替代卵清白蛋白,其余方法与步骤2组均相同。采用行为学评分、鼻腔组织学观察及血清IgE、IL-4、IFN-γ检测综合判断造模是否成功。结果:2组鼻腔激发后行为学评分差异有统计学意义(P<0.05)。对大鼠鼻黏膜进行苏木精-伊红染色,AR组大鼠鼻黏膜符合AR形态学改变,对照组大鼠鼻黏膜变化并不明显。血清总IgE、IL-4、IFN-γELISA检测结果,2组血清中IL-4、IgE含量比较,AR组显著高于正常对照组,差异有统计学意义(P<0.01)。AR组血清中INF-γ含量显著低于正常对照组,差异有统计学意义(P<0.01)。结论:卵清白蛋白致敏成功建立AR大鼠模型,血清总IgE、INF-γ、IL-4含量可作为建立AR动物模型的客观评价指标。
Objective: To investigate the objective and clinical significance of total serum IgE, INF-γ, IL-4 as an experimental rat model of allergic rhinitis (AR) induced by ovalbumin. Methods: SD rats were randomly divided into AR group and control group, with 10 rats in each group. In the AR group, ovalbumin was used as the allergen, and in the control group, the ovalbumin was replaced by normal saline. The rest of the method was the same as that in the second step. Using behavioral score, nasal histological observation and serum IgE, IL-4, IFN-γ test to determine whether the success of modeling. Results: There was significant difference in behavioral score between the two groups after nasal challenge (P <0.05). The nasal mucosa of rats was stained with hematoxylin-eosin, the morphology of nasal mucosa of AR rats was changed, while the changes of nasal mucosa of rats in control group were not obvious. Serum total IgE, IL-4, IFN-γ ELISA test results, two groups of serum IL-4, IgE levels, AR group was significantly higher than the normal control group, the difference was statistically significant (P <0.01). The serum INF-γlevels in AR group were significantly lower than those in normal control group (P <0.01). CONCLUSION: AR rat model was successfully established by ovalbumin sensitization. The contents of total serum IgE, INF-γ and IL-4 could be used as an objective evaluation index for AR animal model.