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目的:研究柴胡疏肝散对肝郁证模型大鼠脑海马CA1、CA3、DG区ERK及其磷酸化免疫阳性神经元的影响。方法:将大鼠分为空白对照组、肝郁模型组、柴胡疏肝散组,运用模具束缚结合孤养的方法制备肝气郁结证候动物模型,采用免疫组化法检测大鼠脑海马CA1、CA3、DG区ERK及其磷酸化免疫阳性神经元的细胞数。结果:柴胡疏肝散可增高ERK1/2、P-ERK在海马CA3、DG区的阳性细胞数。结论:柴胡疏肝散具有抗肝郁作用,其机制可能与通过上调肝郁大鼠海马CA3、DG区ERK1/2、P-ERK信号通路的活动而起到保护受损神经元,改善大脑功能,缓解肝郁症状的作用。
Objective: To study the effect of Chaihu Shugan San on ERK and phospho-immunopositive neurons in hippocampal CA1, CA3 and DG of rats with liver-qi stagnation syndrome. Methods: The rats were divided into blank control group, liver depression model group and Chaihu Shugan powder group. The animal model of liver-qi stagnation syndrome was prepared by using the method of restraining binding with the mold. The immunohistochemical method was used to detect the expression of CA1 , CA3, DG ERK and phosphorylation of immune-positive neurons in the number of cells. Results: Chaihu Shugan Powder can increase the number of positive cells of ERK1 / 2 and P-ERK in CA3 and DG of hippocampus. Conclusion: Chaihu Shugan San has the anti-liver-qi effect. The mechanism may be related to the protection of injured neurons by up-regulating the ERK1 / 2 and P-ERK signaling pathways in CA3 and DG of hippocampus, Function, relieve the symptoms of liver depression.