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[目的]研究2006～2007年河北卢龙地区5岁以下住院腹泻患儿中星状病毒感染的流行特征,确定流行毒株的基因型别和基因组别[方法]收集2006年1月～2007年12月河北卢龙地区住院腹泻患儿粪便标本572份,采用RT-PCR方法检测星状病毒,测序确定其基因型别和基因组别[结果]检测出星状病毒阳性标本47份,检出率为8.2%,患儿的性别比为2.36︰1,年龄为1～24月龄,小于18月龄者占93.6%,,发病以秋冬季多见,10～12月为发病高峰期,74.5%的患儿伴有呕吐或/和发热,42份ORF2基因片段序列和38份ORF1a基因片段序列比对结果为HastV-1,genogroupA。[结论]星状病毒是卢龙地区婴幼儿腹泻的重要病原,HAstV-1为该地区的流行毒株。
[Objective] To study the epidemiological characteristics of astrovirus infection in children under 5 years of age with hospital-acquired diarrhea in Lulong, Hebei Province from 2006 to 2007 and determine the genotype and genotype of the epidemic strains. [Methods] 572 stool samples from hospitalized children with diarrhea in Lulong, Hebei Province in December were detected by RT-PCR and their genotypes and genomic sequences were determined by sequencing. [Results] The positive rate of asci- With a gender ratio of 2.36︰1. The age ranged from 1 to 24 months, while those less than 18 months accounted for 93.6%. The incidence was more common in autumn and winter, with peak incidence in 10-12 months, 74.5% Of children with vomiting and / or fever, 42 ORF2 gene fragments and 38 ORF1a gene fragment sequence alignment results for HastV-1, genogroupA. [Conclusion] Astrovirus is an important pathogen of infant diarrhea in Lulong region. HAstV-1 is a popular strain in this area.