丹参是世界公认的治疗心脑血管病的首选药物,栽培丹参枯萎病发生严重,严重影响栽培丹参产量和质量。针对上述问题,采用形态学和病原学实验手段对从发生枯萎病丹参植株茎和根中分离得到的病原菌进行鉴定,同时根据ITS序列采用PCR的方法进行进一步确定。结果表明丹参枯萎病多发生7—8月高温、多雨季节,栽培一年丹参枯萎病的发生率为10%左右,而栽培三年或者三茬丹参枯萎病的发生率达到了60%~70%。枯萎病会引起丹参根腐,因此生产上易误认为根腐病。形态学鉴定结果表明该病原菌为尖孢镰刀菌,ITS序列也表明其与黄瓜枯萎病菌的ITS序列同源性为99%。因此,作者认为引起丹参枯萎病的病原菌为尖孢镰刀菌。 Salvia is recognized as the world’s first choice of treatment of cardiovascular and cerebrovascular diseases, cultivation of Salvia wilt serious, seriously affecting the cultivation of Salvia yield and quality. In view of the above problems, pathogenic bacteria isolated from the stems and roots of the Salvia miltiorrhiza plant were identified by morphological and etiological experiments and further confirmed by PCR based on ITS sequences. The results showed that the incidence of Salvia miltiorrhiza Fusarium occurred in July and August in the high temperature and rainy season, the incidence of Salvia miltiorrhiza was about 10% in one year of cultivation, while the incidence of Salvia miltiorrhiza wilt was 60% -70% . Fusarium wilt can cause root rot in Salvia miltiorrhiza, so it is easily mistaken for root rot in production. Morphological identification showed that the pathogen was Fusarium oxysporum, ITS sequence also showed ITS sequence homology with Fusarium oxysporum f. Therefore, the author believes that the pathogen causing Salvia blight is Fusarium oxysporum.