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目的评价微小异源二聚体伙伴基因(small heterodi mer partner gene,SHP)基因与新生儿出生体重的关系。方法应用队列研究的方法,收集191例无妊娠合并症及并发症的母血及新生儿脐血标本,利用PCR-RFLP的方法研究SHP基因的单核苷酸多态性,(single nucleotide poly-morphism,SNP)位点rs7504的基因多态性。结果(1)随出生体重的增加新生儿携带C等位基因和TC+CC基因型频率增加,且有统计学差异(P=0.004;P=0.005);孕妇C等位基因和TC+CC基因型频率亦随新生儿出生体重增加而增加,有统计学差异(P=0.013,P=0.013)。(2)此位点携带C等位基因者新生儿体重增加,携带TC+CC基因型的新生儿比携带TT基因型的新生儿体重平均增加246.3g[(3658.7±400.9)g和(3412.4±444.4)g,P=0.005)]。同时孕妇携带C等位基因者其分娩新生儿体重比不携带C等位基因孕妇分娩的新生儿体重平均增加210.3g[(3418.6±449.0)g和(3628.9±405.5)g,P=0.011)]。(3)比较脐血TC+CC基因型与TT基因型的临床参数,发现SHP基因与血糖和胰岛素不相关;与母亲的体重、孕前BMI、父亲的身高、体重相关。孕妇携带TC+CC基因型者体重和孕前BMI略有增加,但无统计学差异;且与血糖和胰岛素水平无明显相关。(4)多因素分析结果表明孕妇身高、孕期增重、孕前BMI、分娩孕周、脐血胰岛素、母血胰岛素水平均与新生儿体重相关,调整以上因素之后,脐血SHP基因仍与新生儿体重显著相关(P=0.0354),母血SHP基因与新生儿体重无明显相关(P>0.05)。结论SHP基因与新生儿出生体重相关,在胎儿宫内发育中起一定的作用。
Objective To evaluate the relationship between small heterodimer partner gene (SHP) gene and birth weight in neonates. Methods A cohort study was conducted in 191 maternal and neonatal umbilical cord blood samples of non-pregnant women with complications and complications. Single nucleotide polymorphism (SHP) gene polymorphism was examined by PCR-RFLP. morphism, SNP) rs7504 gene polymorphism. Results (1) The frequencies of C allele and TC + CC genotypes increased with the increase of birth weight, and there was a significant difference (P = 0.004; P = 0.005). The C allele and TC + CC gene The frequency also increased with the birth weight of newborns, with statistical difference (P = 0.013, P = 0.013). (2) The newborn infants born with C allele at this locus had an increased body weight. The newborns with TC + CC genotype had an average increase of 246.3 g [(3658.7 ± 400.9) g and (3412.4 ± 444.4) g, P = 0.005)]. At the same time, the weight of newborn infants born with C allele increased 210.3g [(3418.6 ± 449.0) g and (3628.9 ± 405.5) g, P = 0.011) than the infants born without pregnant C allele . (3) The clinical parameters of TC + CC genotype and TT genotype in cord blood were compared. It was found that the SHP gene was not related to blood glucose and insulin; it was related to the mother’s body weight, BMI before pregnancy, father’s height and weight. Pregnant women carrying TC + CC genotype had a slight increase in body weight and BMI before pregnancy, but no significant difference; and no significant correlation with blood glucose and insulin levels. (4) The results of multivariate analysis showed that the height of pregnant women, weight gain during pregnancy, BMI before pregnancy, gestational weeks of pregnancy, serum umbilical insulin and maternal insulin were all related to birth weight. After adjustment for the above factors, SHP gene in umbilical cord blood was still associated with neonatal Body weight was significantly correlated (P = 0.0354). There was no significant correlation between maternal SHP gene and newborn body weight (P> 0.05). Conclusion The SHP gene is associated with the birth weight of newborns and plays a role in intrauterine development.