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目的:观察、比较氟桂嗪与尼莫地平对慢性退变中神经元的保护作用。方法:21只生后2d新生大鼠,切断右侧坐骨神经作为实验侧,左侧为正常对照侧。术后第5天,氟桂嗪组皮下注射氟桂嗪(25μg/g体重),尼莫地平组皮下注射尼莫地平(20μg/g体重),对照组注射等量溶剂,连续14d,然后取L4～6脊髓,在光镜下观察并计数脊髓前角神经元。结果:在神经元慢性退变过程中,氟桂嗪组与尼莫地平组可以分别平均支持78.3%和58.1%神经元存活,统计学上以前者占优势,且两者与对照组相比(存活53.2%)差异显著。氟桂嗪组实验侧脊髓前角神经元核团改变较小,其胞体萎缩程度较轻。结论:氟桂嗪与尼莫地平对慢性退变过程中神经元均有保护作用,而氟桂嗪的保护作用更显著。
Objective: To observe and compare the protective effect of flunarizine and nimodipine on neurons in chronic degeneration. METHODS: Twenty-one newborn rats were born 2 days after birth. The right sciatic nerve was cut off as experimental side and the left side was normal control side. On day 5 after surgery, flunarizine (25 μg / g body weight) was injected subcutaneously, nimodipine (n = 20 μg / g body weight) was injected subcutaneously in the nimodipine group, and the control group was injected with the same amount of solvent for 14 days L4 ~ 6 spinal cord, under the light microscope to observe and count spinal cord anterior horn neurons. Results: During chronic degeneration of neurons, flunarizine group and nimodipine group could respectively support 78.3% and 58.1% neuron survival on average, the former was predominant in statistics, and both were significantly lower than those in control group Survival 53.2%) significant difference. Flunarizine group experimental spinal cord anterior horn neurons smaller change, the extent of their cell body atrophy lighter. Conclusion: Both flunarizine and nimodipine have protective effects on neurons during chronic degeneration, while flunarizine has a more protective effect.