建立了同时测定茶叶中柠檬黄、日落黄、苋菜红、胭脂红、诱惑红、亮蓝6种合成着色剂的高效液相色谱法。样品经乙酸乙酯和正己烷清洗后,用去离子水提取,提取液供高效液相色谱仪测定,外标法定量。本方法 6种合成着色剂在0~100 mg/L浓度范围内线性关系良好,相关系数r2>0.999,回收率80.0%~90.2%,相对标准偏差均小于5%。 A high performance liquid chromatography (HPLC) was developed for the simultaneous determination of six kinds of synthetic colorants including lemon yellow, sunset yellow, amaranth, carmine, temptation red and brilliant blue in tea. The sample was washed with ethyl acetate and n-hexane, extracted with deionized water, the extract was determined by HPLC, external standard. The linearity of the 6 synthetic colorants in this method ranged from 0 to 100 mg / L with a correlation coefficient of r2> 0.999 and a recovery of 80.0% to 90.2% with relative standard deviations less than 5%.