高效液相色谱法中溶质在色谱柱上的保留行为通常由容量因子k′表示,k′值的确定须由色谱柱死体积V_0计算得到,所以V_0测定尤为重要。关于反相离子对色谱中死体积研究较少,Bidlingmeyer用甲醇作探剂测定反相离子对色 The retention behavior of solutes on a chromatographic column in HPLC is usually expressed by the capacity factor k ’. The k’ value must be calculated from the column void volume V_0. Therefore, the V_0 determination is particularly important. On the reverse phase ion pair chromatography in the study of dead volume less, Bidlingmeyer methanol as a probe for the determination of inverse ion pairing